Lamina joint inclination assay
WebJul 3, 2024 · Lamina joint inclination assay. Seeds are sterilized with 2.5% sodium hypochlorite solution, soaked in the dark at 37 °C for 2–3 days, and then germinated in a light incubator under long-day conditions (16L/8D, 28 °C, 70% humidity) for 3 days. ... Lamina inclination assays and expression analysis of BR-related gene between WT … WebBrassinosteroid (BR)-induced rice (Oriza sativa L.) lamina joint (RLJ) inclination and its relationship to indole-3-acetic acid (IAA) and ethylene were investigated using BR …
Lamina joint inclination assay
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WebJan 29, 2016 · For the lamina inclination assays, 1 µl of ethanol containing a series of concentrations of BRs, IAA or Brz was spotted onto the tips of the lamina of 5- or 6-day-old seedlings. C-22-hydroxylated and 6-deoxo brassinosteroids were identical to those described by Nakamura et al. (2009). WebMar 18, 2024 · Scanning electron microscopy revealed that the expansion of cell size in the adaxial side of the lamina joint was responsible for the increase in leaf angle in osbhlh079-D. The expression of...
WebJan 27, 2024 · Lamina joint inclination test To test the sensitivity of ZH11, rel1, rip2, RIP2 -OE, and rel1rip2 to 24-epibrassinolide (24-epiBL) in a lamina joint inclination experiment, ethanol containing 0, 10, 100, or 1000 ng of 24-epiBL was applied to the lamina joint of the first leaf of rice seedlings. WebTo show that lamina joint inclination of osbul1 is less-sensitive than WT to exogenous brassinolide (BL) treatment in the lamina joint inclination bioassays, we applied the protocol presented below. The protocol focuses on: (1) how to prepare rice samples for the assay, and (2) how to treat BL exogenously. Finally, we have added a result ...
WebJul 20, 2024 · This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our … WebApr 14, 2024 · Furthermore, a BR-induced lamina joint inclination assay (Wada et al., 1984) confirmed that the sensitivity of OsMYB7 overexpressors and the osmyb7-1 mutant are not altered by BR treatments, compared to the WT (Supplementary Figure S6). Taken together, these findings suggest that OsMYB7 is involved in the regulation of leaf angles …
WebFeb 9, 2024 · Lamina joint inclination assay. The lamina joint test was performed following a well-established procedure (Li et al., 2024b). After floating on distilled water for 24 h, the entire segments consisting of 1 cm of leaf blade and sheath were incubated in 2.5-mM maleic acid potassium solution containing 1-µM BL or dimethyl sulfoxide (DMSO; mock ...
WebFeb 5, 2024 · For lamina joint inclination assay, sterilized rice seeds were grown in the dark at 30 °C for 9 days. We obtained equally sized samples by excising approximately 2 … maria balentoWebNov 29, 2024 · Our previous studies by analyzing the global transcriptome of developing lamia joint showed that gene leaf inclination3 ( LC3, Os06g39480) is down-regulated from stage 2 to stage 6 during lamina joint development. Further analysis of the corresponding knockout mutant, lc3, revealed the obviously increased leaf angles under LC3 deficiency … cura per aumentare difese immunitarieWebBrassinosteroid (BR)-induced rice (Oriza sativa L.) lamina joint (RLJ) inclination and its relationship to indole-3-acetic acid (IAA) and ethylene were investigated using BR isolated from beeswax. The effect of BR on RLJ inclination was time- and concentration-dependent. Etiolated lamina were more sensitive to BR than green lamina. The BR-induced … cura per cistite ricorrenteWebNov 21, 2006 · Lamina inclination assay is a classical and specific method to determine the effect of BR in rice 18. Both BR-insensitive and -deficient rice mutants display erect leaves 16 , 19 , 20 . cura per fuoco di sant\u0027antonioWebMay 12, 2024 · Lamina joint develops along with the leaf blade and sheath, and fully developed lamina joint supports the leaf with an appropriate inclination for light capture and high-efficient photosynthesis. cura perimeters settingWebFigure 3. BR-induced lamina joint inclination in WT (Hwayoung cultivar) and OsBUL1 knock-out mutant (osbul1) rice. The photo was taken after BL (10-6 M) treatment for 2 … maria ballenWebMar 8, 2024 · The leaf bending caused by extracts from Distylium racemosum in the rice lamina inclination assay is one of the earliest reports of brassinolide ( 1) activity in plants [ 3 ]. In this assay, 1 caused dose-dependent swelling of the adaxial cells in the joint between the leaf blade and sheath of etiolated rice seedlings. maria baldini potermin and associates