Flag-tag mouse monoclonal antibody
WebTHE™ DYKDDDDK Tag Antibody, mAb, Mouse THE™ DYKDDDDK Tag Antibody, mAb, Mouse recognizes DYKDDDDK tags localized at the C-terminal, N-terminal and internal region of DYKDDDDK tagged fusion proteins. Product Details Citations Documents Overview Applications Properties Examples Background For laboratory research use only. WebMouse Antibody Monoclonal. Introduction. A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. …
Flag-tag mouse monoclonal antibody
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WebThe FLAG™ Tag (peptide sequence DYKDDDK) is a short, ... The membrane was probed with DYKDDDDK Tag Monoclonal Antibody (L5), Alexa Fluor 488 (Cat. No. MA1-142-A488) at a dilution of 1:500 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using a fluorescence imaging … WebSpecificity: Flag-Tag Mouse Monoclonal antibody detects endogenous levels of C-terminal, internal, and N-terminal Flag-tagged proteins. Immunogen: A synthesized peptide. Western blot analysis of Flag-tagged protein expression in (1) 293T cell lysate; (2) 293T cell transfected with Flag-tagged protein lysate. β-Actin Rabbit Polyclonal Antibody
WebNeuritin represents a neurotrophic factor that is not only important in neuronal development and plasticity but also impacts endothelial angiogenesis, cell migration, tumor growth and … WebMonoclonal ANTI-FLAG ® M1 antibody produced in mouse is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.coli cells. …
WebA FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Web> 无血清快速细胞冻存液. QuickFreezing-M. QuickFreezing-2*M
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WebNeuritin represents a neurotrophic factor that is not only important in neuronal development and plasticity but also impacts endothelial angiogenesis, cell migration, tumor growth and the production of antibodies by B cells. We established monoclonal mouse anti-mouse neuritin antibodies by immunizing knock-out mice with two different neuritin-derived … nail salon sleafordWebHost Species Mouse; Clonality Monoclonal; Purification Affinity purification using immunogen. Applications WB,IF IP; Species Reactivity Hu Ms Rt; Specificity The Flag … medium hairstyles for thick hair square faceWebFlag Tag monoclonal antibody CSB-MA000021M0m was produced in the mouse immunized by using the DYKDDDDK(Flag) synthetic peptide conjugate to KLH as the … medium hairstyles for long facesWebThe FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. A FLAG tag can be used in many different assays that require recognition by an antibody. nail salons junction city ksWebThe MA1-142 antibody (clone L5) has been successfully used as a detection antibody in a direct ELISA with FLAG-tagged protein-coated plates. Similar Epitope as the FLAG tag … medium hairstyles for teenage girlsWebDetection was performed with ANTI-FLAG® M2 monoclonal primary antibody, anti-mouse-HRP secondary antibody, and ECL™ chemiluminescent substrate. Figure 3. Comparative Sensitivity of 3xFLAG®. Each protein fusion tag was cloned separately on the C terminal of GST. The purified proteins were diluted from 1 ug to 0.01 ng and analyzed. medium hairstyles for round faces over 50WebMBP Tag Mouse Monoclonal Antibody (HRP Conjugated) The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Our anti-MBP monoclonal antibody provides a simple solution to detect the expression of protein ... nail salons in windsor colorado